RNA

Part:BBa_K3760005

Designed by: Ha Buer   Group: iGEM21_LZU-CHINA   (2021-10-01)


RfxCas13d-hACE2-sgRNA1

The sgRNA is an oligonucleotide chain, it can combine with Cas13 protein, and guide it to find the target RNA sequences. It consists of two parts, DR sequence and spacer. The DR sequence is determined by Cas protein, and different Cas proteins have different DR sequences. And the spacer is determined by a specific target. Here the sgRNA is corresponding to RfxCas13d and hACE2 , which could combine with RfxCas13d protein and guide it to target hACE coding RNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None